2. How to verify the normal operation of fluorescence detection for PCR instruments?
2026-03-24 11:17
To judge whether the PCR instrument works normally, consider the following aspects:
1. Check if the instrument can be turned on/off normally and connected to software stably;
2. Verify if the runtime for the same experiment is consistent with usual to judge the normal operation of the heating and cooling module;
3. Check if the amplification curve shows a typical S-shape. An abnormal zigzag rising curve may indicate issues with the heated lid, fluorescence detection device or voltage;
4. If the fluorescence intensity is significantly lower than usual, consider replacing the instrument light source (especially halogen lamps with a lifespan of about 2000h);
5. If the fluorescence intensity of one or several wells is significantly higher than others, the well slots or detection optical path may be contaminated by fluorescence. Clean and calibrate the well slots before retesting.
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