1.What to do if the laboratory is contaminated with PCR amplification products?

2026-03-24 11:17


Amplification product leakage can lead to severe laboratory contamination. Follow these steps to eliminate contamination:
① Prioritize ventilation to ensure the smooth diffusion of amplification product fragments;
② Treat suspicious equipment by wiping or soaking with 1mol/L hydrochloric acid to depurinate residual DNA;
③ Use UV irradiation with a wavelength of 254/300 nm (Note: UV only works for long fragments over 500 bp with little effect on short ones);
④ If contamination persists for a long time, replace PCR reagents with products from another manufacturer for testing.